Determinants for Membrane Fusion Induced by Cholesterol-Modified DNA Zippers
Identifieur interne : 002A35 ( Main/Exploration ); précédent : 002A34; suivant : 002A36Determinants for Membrane Fusion Induced by Cholesterol-Modified DNA Zippers
Auteurs : Gudrun Stengel [Suède] ; Lisa Simonsson [Suède] ; Richard A. Campbell [Suède] ; Fredrik Ho O K [Suède]Source :
- The Journal of Physical Chemistry B [ 1089-5647 ] ; 2008.
Abstract
Intracellular membrane fusion is coordinated by membrane-anchored fusion proteins. The cytosolic domains of these proteins form a specific complex that pulls the membranes into close proximity. Although some results indicate that membrane merger can be accomplished solely on the basis of proximity, others emphasize the importance of bilayer stress exerted by transmembrane peptides. In a reductionist approach, we recently introduced a fusion machinery built from cholesterol-modified DNA zippers to mimic fusion protein function. Aiming to further optimize DNA-mediated fusion, we varied in this work length and number of DNA strands and used either one or two cholesterol groups for membrane anchoring of DNA. The results reveal that the use of two cholesterol anchors is essential to prevent cDNA strands from shuttling to the same membrane, which leads to vesicle release instead of membrane merger. A surface coverage of 6−13 DNA strands was a precondition for efficient fusion, whereas fusion was insensitive to DNA length within the tested range. Besides lipid mixing, we also demonstrate DNA-induced content mixing of large unilamellar vesicles composed of the most abundant cellular lipids phosphatidylcholine, phosphatidylethanolamine, cholesterol, and sphingomyelin. Taken together, DNA-mediated fusion emerges as a promising tool for the functionalization of artificial and biological membranes and may help to dissect the functional role of fusion proteins.
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DOI: 10.1021/jp802005b
Affiliations:
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Phys. Chem. B</title><idno type="ISSN">1089-5647</idno><idno type="eISSN">1520-5207</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2008</date><date type="published">2008</date><biblScope unit="vol">112</biblScope><biblScope unit="issue">28</biblScope><biblScope unit="page" from="8264">8264</biblScope><biblScope unit="page" to="8274">8274</biblScope></imprint><idno type="ISSN">1089-5647</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1089-5647</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Intracellular membrane fusion is coordinated by membrane-anchored fusion proteins. The cytosolic domains of these proteins form a specific complex that pulls the membranes into close proximity. Although some results indicate that membrane merger can be accomplished solely on the basis of proximity, others emphasize the importance of bilayer stress exerted by transmembrane peptides. In a reductionist approach, we recently introduced a fusion machinery built from cholesterol-modified DNA zippers to mimic fusion protein function. Aiming to further optimize DNA-mediated fusion, we varied in this work length and number of DNA strands and used either one or two cholesterol groups for membrane anchoring of DNA. The results reveal that the use of two cholesterol anchors is essential to prevent cDNA strands from shuttling to the same membrane, which leads to vesicle release instead of membrane merger. A surface coverage of 6−13 DNA strands was a precondition for efficient fusion, whereas fusion was insensitive to DNA length within the tested range. Besides lipid mixing, we also demonstrate DNA-induced content mixing of large unilamellar vesicles composed of the most abundant cellular lipids phosphatidylcholine, phosphatidylethanolamine, cholesterol, and sphingomyelin. Taken together, DNA-mediated fusion emerges as a promising tool for the functionalization of artificial and biological membranes and may help to dissect the functional role of fusion proteins.</div></front></TEI><affiliations><list><country><li>Suède</li></country></list><tree><country name="Suède"><noRegion><name sortKey="Stengel, Gudrun" sort="Stengel, Gudrun" uniqKey="Stengel G" first="Gudrun" last="Stengel">Gudrun Stengel</name></noRegion><name sortKey="Campbell, Richard A" sort="Campbell, Richard A" uniqKey="Campbell R" first="Richard A." last="Campbell">Richard A. Campbell</name><name sortKey="Ho O K, Fredrik" sort="Ho O K, Fredrik" uniqKey="Ho O K F" first="Fredrik" last="Ho O K">Fredrik Ho O K</name><name sortKey="Ho O K, Fredrik" sort="Ho O K, Fredrik" uniqKey="Ho O K F" first="Fredrik" last="Ho O K">Fredrik Ho O K</name><name sortKey="Ho O K, Fredrik" sort="Ho O K, Fredrik" uniqKey="Ho O K F" first="Fredrik" last="Ho O K">Fredrik Ho O K</name><name sortKey="Simonsson, Lisa" sort="Simonsson, Lisa" uniqKey="Simonsson L" first="Lisa" last="Simonsson">Lisa Simonsson</name><name sortKey="Stengel, Gudrun" sort="Stengel, Gudrun" uniqKey="Stengel G" first="Gudrun" last="Stengel">Gudrun Stengel</name><name sortKey="Stengel, Gudrun" sort="Stengel, Gudrun" uniqKey="Stengel G" first="Gudrun" last="Stengel">Gudrun Stengel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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